Re: In vitro seed culture

From: Guy Van der Kinderen (Guy.VanderKinderen@rug.ac.be)
Date: Wed Jun 07 2000 - 03:44:07 PDT

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Date: Wed, 7 Jun 2000 11:44:07 +0100
From: "Guy Van der Kinderen" <Guy.VanderKinderen@rug.ac.be>
To: cp@opus.hpl.hp.com
Message-Id: <aabcdefg1743$foo@default>
Subject: Re: In vitro seed culture

Dear Miguel,

> A brief question:
>
> What concentration of Sodium hypochlorite do people use most successfully
> when disinfesting seed for in vitro culture?
>
> I'm familiar with timing, etc... but I've 1.5% hypochlorite, which I can
> further dilute.

I like to use a saturated solution of alkaline calciumhypochlorite,
with the addition of 1 % Tween 80 (or Tween 20, or another
surfactant), for at least 15 minutes (use the clear solution when the
excess of solid has precipitated after vigorous shaking). It's more
reproducible, because (commercial) solutions of sodium
hypochlorite may loose chlorine upon storage.

Good sowing,

Guy

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