Withdrawal

Jan Schlauer (zxmsl01@studserv.zdv.uni-tuebingen.de)
Sat, 8 Jan 1994 09:29:08 +0100

Dear tissue culture gurus:

>After gleaning through the archives, this is what I came up with for
>recommended media (these are mostly due to Jan S.):
>
> _Pinguicula_
> Carroll's media (ICPN v11 n4 12/82 pp. 93-96)
> Murashige-Skoog media diluted 1:5, sucrose 20g/L

The first can be recommended for ALL _Pinguicula_ species tested so far
(ca. 30 spp. of different subgenera and sections).

> Terrestrial _Utricularia_
> Carrol's media

I can recommend this media ONLY for the large-leaved species _U.alpina_,
_U.longifolia_, and _U.calycifida_.

!!! * W A R N I N G * !!!

DO NOT USE CARROLL's MEDIA FOR _Utricularia_ sect.Pleiochasia!
IT WILL ALMOST CERTAINLY KILL YOUR PLANTS (at least young seedlings) RAPIDLY.
It did so for me with several species 8-(
I haven't yet any solution to the problems concerning these, some other
terrestrial species, and most _Genliseae_. _G.pygmaea_ seems to be very
tolerant, _G.violacea_ grows and flowers (!!) in 1/5 MS media (J.Nerz,
pers. comm.).

> Aquatic _Utricularia_
> Carroll's media (no agar)
> Pringsheim & Pringsheim media

The latter being the better one. The reference is:
Amer.J.Bot.49:898-901 (1962). Addition of sucrose may be helpful (the
original formula doesn't contain sucrose).

Kind regards and happy growing!
Jan