>I recently heard you say you use .7% mixture of sigma agar. Just curious what
>>other nutrients you add to that
Of course, the salts and vitamins of the media discussed have to be added!
> and if you have experimented with any antibiotics to keep fungal growth down.
Yes, there has been some experimenting around with various antibiotics. The
result was, however, not at all satisfactory. The method recommended is
proper disinfection of the material. As a rule, once there is a fungal
infection, antibiotics won't be too effective. If the cultures are
"clean" anyway, antibiotics are of no benefit (even worse: you will
realize infections later than without antibiotics).
Kind regards
Jan