> I was wondering if some of you out there could give me some advice on
> tissue culture of Drosera (D. capensis and D. binata specifically)?
> I am an undergraduate student taking a class on tissue culture and my
> professor has given us a project where we choose a plant (such as a
> house plant) to reproduce through this method. One piece of
> information I need right away is what culturing media is appropriate
> so that I can get it ready ASAP (my teacher gave us two days to find
> this out!). I have not been able to find any sources on tissue
> culturing Drosera, so any information you have or you could direct me
> to would be greatly appreciated.
It has been some time since we've discussed TC formulas. I'm also just
a beginner, but here is a list summarizing what I know (with great
appreciation to Jan Schlauer, Andreas Wistuba, and Tom Hillson for their
input!) If anyone else can add to this list, please do!
---------------------------------------------------------
Summary of CP Tissue Culture Formulas and References by
Family and Genus:
Sarraceniaceae {DUMORT.}
Darlingtonia {TORR.}
Heliamphora {BENTH.}
Sarracenia {L.}
- 2/3 Knudsen C(*) with BAP, ABA for mult and root
- 1/6 MS, is second choice
Byblidaceae {DOMIN}
Byblis {SALISB.}
- Sigmas modified MS (1/2 x macro-, 1 x micro-)(M0153) + BAP,
IBA sometimes vitrification trouble with B.gigantea
- Bunn 1985. Australian Horticulture. 83(5):103
Cephalotaceae {DUMORT.}
Cephalotus {LABILL.}
- Sigmas modified MS (1/2 x macro-, 1 x micro-)(M0153)+BAP, IBA
- In vitro propagation of Cephalotus follicularis (Australian
Pitcher Plant). HortScience 14, 521-513
Droseraceae {R.A.SALISB.}
Aldrovanda {L.}
Dionaea {SOLAND. ex ELLIS}
- Hutchinson 1984. Scienta Horticulturae 22:189-194.
- Beebe 1980. Bot. Gaz.141(4):396-400.
- Parliman et al. 1982. J.Amer.Soc.Hort.Sci. 107(2):305-310.
- Parliman et al. 1982. J.Amer.Soc.Hort.Sci. 107(2):310-316.
Drosera {L.}
- 2/3 Knudsen C (*)
- Janssens 1986. Med.Fac.Landbouww.Rijksuniv.Gent. 51(1):61-66.
- Anthony, J. (1992). In vitro propagation of Drosera spp.
HortScience 27, 850.
Drosophyllum {(L.) LINK}
Nepenthaceae {DUMORT.}
Nepenthes {L.}
- 2/3 Knudsen C(*)with 0.2-2mg/L BAP for mult. Rooting horm.
not neces.
- Anderson's may be used also (N. ephippiata likes it).
- Lowland only: (DeKanel) .5 MS, 20g/L Sucrose, 6g/L agar.
w/ 0.1-0.2 BAP multiplying
w/ 2mg/L IBA rooting
Dioncophyllaceae {(ENGL. & GILG) AIRY-SHAW}
Dioncophyllum {BAILL.}
Habropetalum {AIRY SHAW}
Triphyophyllum {AIRY SHAW}
Lentibulariaceae {L.RICH.}
- Bill Carroll's media (ICPN v11 n4 12/82 pp. 93-96) <JS>
- 1:5 MS
Genlisea {ST.HIL.}
- 1/5 MS for G.pygmaea
Pinguicula {L.}
- Adams et al. 1979. HortScience 14(6):701-702.
Utricularia {L.}
-Pringsheim & Pringsheim esp. for aquatics
Amer.J.Bot.49:898-901 (1962)
-Carrols's ONLY for large-lvd. sp: U.alpina, longifolia,
calycifida
*= You may add 37,26mg/l Na2EDTA and 27,8 mg/l FeSO4 x 7H2O.
I always add the MS-vitamins to the Knudsen C medium.
General References:
1. Achieving the sterile state for home tissue culture, Part I,
Brian Johnson, CPS Journal 14, 18-19.
2. Achieving the sterile state for home tissue culture, Part II,
Brian Johnson, CPS Journal 16, 9-10.
3. Tissue culture of carnivorous plants at Oxford. Steve Woodward, et
al. CPS Journal 15, 16-19.
4. Tissue culture of carnivorous plants. Gareth Davies et al. CPS
Journal 12, 17-20.
5. In Vitro propagation of the Butterwort Pinguicula moranensis,
Richard Adams et al. HortScience 14(6), 701-702.
6. Tisserat et al. (1992). Microwave sterilization of plant tissue
culture media. HortScience 27, 358-361.
7. Plants from Test Tubes - an introduction to micropropagation,
Lydiane Kyte, 1987 Timber Press (Oregon), ISBN 0-88192-040-1
-- Rick Walker