Re: IN VITRO stuff

Bob Beer (bbeer@u.washington.edu)
Mon, 9 Jan 1995 07:14:50 -0800 (PST)

It seems that if we are dealing with species and not hybrids where exact
hybrid traits are wanted, tissue culture should be looked upon as, if not
a last resort, then as less desirable. Seed is more work, especially in
plants that are dioiceous like Nepenthes, but the genetic diversity is
just as important in culture as it is in the wild. And who's to say that
there will never be opportunities to reintroduce some of these species
back into the wild? It is not the same as wild plants, but there is
better chance of succeeding if we have the greatest amount of genetic
material possible.

I have been told by rose experts that many of the old varieties that have
been reproduced by cloning over the years are destined to die out
eventually; it is "tired" genetic material and it becomes less viable
each time it is cloned. We are talking hundreds or more years (some
roses varieties have been in cultivation since egyptian times) but the
effects are becoming evident. We certainly want to make sure we don't
put individual species into this position.

>From my understanding, however, tissue culture, although it produces a
high degree of uniformity, is not *completely* "fool-proof" in producing
all clones; some degree of mutation does occur. I know of cases where
tissue culture of a particular variegate form or color of a plant comes
out with a certain small percentage reverting to non-variegated, or a
different color flower. (I realize these numbers are extremely small though)