Re: Tissue Culture Tutorial & Media
Chris Marsden (100620.2156@compuserve.com)
07 Feb 96 09:18:38 EST
Everybody,
I have spent some time over the last month or so preparing a
list of
tissue culture media as several people have expressed interest in it.
On completion I accessed the internet CP web site and looked at the CP
tutorial. The comprehensive list of media and techniques there is much
fuller than the list that I had created and I feel it would be extremely
useful to beginners (and maybe more advanced hobbyists as well) to go
there and spend a few minutes downloading the tutorial. This, of
course, has made my list of media redundant so I won't post it onto the
list. However, there are two things that most people know but could be
of some use to those in the same boat as me, i.e contemplating Tissue
Culture.
1) Heliamphora species, notorious amongst many culturists, can be fairly
easily cultured in the same conditions as Highland Nepenthes conditions
(i.e. 2/3 Knudson C Orchid medium) with the same media.
2) Heliamphora and Nepenthes species can survive quite happily without any
vitamins. However, having 'talked' with several veteran culturists, apparently
Neps and Heliamphora sp. are best grown as for the Nepenthes entry in the TC
tutorial on the WWW but with 0.1mg/l NAA.
I hope this may be of some use to those of you that are just starting TC
of CP. If anyone notices any mistakes or has got ANYTHING to add to my
comments, please contact me at 100620,2156@compuserve.com
Toby Marsden