tissue culturing Sarracenias
Peter Cole (carnivor@bunyip.demon.co.uk)
Thu, 15 Aug 1996 22:47:17 GMT
A question for any of the list's resident TC gurus - I've tried and
tried, but I can't seem to get Sarracenia leaf tissue or rhizome borings
sterile using bleach alone, even with concentrations up to 20% and times
up to 30 minutes. Anyone know a good protocol? Should I pretreat the
plants in some way perhaps? I've tried dosing them with systemic fungicide
(Benlate,) 2 or 3 days before meristemming, but this doesn't seem to help.
I'm using 1/6 MS and some plus 1mg/L IBA, but that's no help if I can't get
the inocula sterile.
If anyone's interested, I've added some more TC info on my website, and
Mike Salmon's TC kits are now available.
TIA for any info,
Peter
snail:Peter Cole,17 Wimmerfield Cr. :mailto:carnivor@bunyip.demon.co.uk
Killay, SWANSEA SA2 7BU, WALES :http://www.angel.co.uk/flytrap/index.htm
vox:+44 1792 205214 :TC Kits available - details on home page