> Attention tissue culture buffs. . . in Rick Walker's Tissue Culture slide
> show (walker@opus.hpl.hp.com) reference is made to "2iP" in the section on
> CP Tissue Culture Formulas and References. Can anybody tell me exactly
> what this is?
2iP is a hormone (used, incidentally, in the Murashige and Skoog
medias).
It's full name is:
6-(y,y,dimethylally-amino)-purine.
It is a cytokinin, and is more powerful than either Kinetin (K) or
6-benzylaminopurine (BAP), the other 2 most popular cytokinins used for
microprop work.
As a side note, it is used in "Stage II" of the TC process
(multiplication) in conjunction with a low auxin content, to promote
shoots, and make the explants multiply rapidly. Auxins, when used in
conjunction with a low cytokinin content, are used in "Stage III"
(rooting) to promote roots.
Hope this helps,
Toby