RE: CP TC media problems

From: Guy Van Der Kinderen (Guy.VanDerKinderen@rug.ac.be)
Date: Thu Sep 18 1997 - 23:46:01 PDT

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Date: Fri, 19 Sep 1997 08:46:01 +0200 (MET DST)
From: Guy Van Der Kinderen <Guy.VanDerKinderen@rug.ac.be>
To: cp@opus.hpl.hp.com
Message-Id: <aabcdefg3593$foo@default>
Subject: RE: CP TC media problems

Carl Mazur wrote:

> The last 3 batches of media I have made have had the consistancy of
> runny pea soup when cooled.

Hi Carl,

Did you check and adjust the pH of your medium before adding agar?
I don't know the exact value from my head here and now, but a low pH value
(< 5?) may prevent hardening of agar gels. Also cooking of agar in a
medium with a low pH may have the same result.

Kind regards,

Guy VdK

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