Re: micropropagation of Pinguicula and Drosera

From: Peter Cole (carnivor@flytrap.demon.co.uk)
Date: Mon Mar 23 1998 - 02:39:07 PST

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Date: Mon, 23 Mar 1998 10:39:07 +0000
From: "Peter Cole" <carnivor@flytrap.demon.co.uk>
To: cp@opus.hpl.hp.com
Message-Id: <aabcdefg1000$foo@default>
Subject: Re: micropropagation of Pinguicula and Drosera

Jon Lindstrom <tranell@omni.cc.purdue.edu> writes:

> I have plants of Pinguicula sharpii and Drosera capensis in culture
> (from seed). Has anyone on the list been successful propagating P.
> sharpii in tissue culture? I realize that D. capensis has less value
> but it is a good plant for student experiments in tissue culture.
> Does anyone have advice on the best hormone concentrations for
> multiplication in culture. Thanks for the help.

        20% strength MS medium with 100% vitamins and 20g/L sucrose does the
        trick for most Drosera (including capensis,) and Mexican Pinguicula
        (though I've not tried sharpii.) I find 65% Knudsons C a good
        alternative to the MS too (vitamins and sucrose as above.) No
        need for buffering or hormone tweaking. I've a feeling capensis
        would grow in almost anything! :-)

Happy cloning,

Peter

+++ Peter Cole, 17 Wimmerfield Cr.,Killay,SWANSEA SA27BU,WALES,UK +++
mailto:carnivor@flytrap.demon.co.uk - http://www.flytrap.demon.co.uk/
++++ Carnivorous Plants, seeds and tissue culture kits for sale ++++

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