Date: Sun, 24 Jan 1999 00:43:58 +0900 (JST) From: takaicp@sh0.po.iijnet.or.jp To: cp@opus.hpl.hp.com Message-Id: <aabcdefg202$foo@default> Subject: Re: Utricularia pollination and in-vitro propagation
Dear Jan-san and Loyd-san,
Jan-san,
Thank you very much for the infomation.
> > Please contact me directly.
> The small talk above may be of interest for a few others on this list,
> so I posted it here. The rest of the listeners are (like always) free
> to make full use of their delete keys.
No problem. As you know,I can not catch up with the progress speed of the
conversation on this list. And also, I wanted to suppress to make this
list dirty with my terrible English to the minimum.
Loyd-san Jan-san,
> > There are other
> > reports of in-vitro plants 'going wrong' in the UK -
> > Pinguicula which constantly divide but never flower, others
> > which do not know what time of year it is and form winter
> > rosettes in mid summer.
>
> This is entirely normal in vitro.
>
> >. I would be interested to know
> > whether these observations are purely anecdotal or whether
> > in-vitro cultivation can lead to subsequent problems when
> > the plants are unflasked.
>
> Both legend and truth seem to be involved here.
The such problem occurs to the other plant.
For example, Cut Gerberas
The experienced tissue culture company which produces Cut Gerberas always
pays the such problem an attention. They become careful when
extraordinarily well propagating clone emerges in vitro. It often becomes
like Peter Pan Heliamphora which you described. Then, it constantly divides
but never flower like Pinguicula which you described.
The experienced company deletes such clone before sale.
I don't know whether or not the phenomenon is gene mutation by in-vitro
propagation. It may be that the phenomenon causes by operating the gene
which is usually sleeping. To make the operation of the gene sleep, some
stimulation may be necessary.
Cut Gerbera farmers which bought such plants tries to solve the problem in
following method.
They choose one grown-up point in the plant which repeats division. They
remove the other grown-up points. For a while, they repeat the operation.
When the plant begins to produce flowers normally, they stop the operation.
After that, they maintain several grown-up points like the normal other
plants.
I have seen Peter Pan Sarracenia seedlings (not from in-vitro). The method
of Cut Gerbera farmers was effective.
In any case,
All of the seeds which collected from one pod aren't always sound. Very
weak clone may survive by In-vitro culture. It might be dead when it was
sown on soil.
The bud mutation which is found out in the large breeding field occurs in
the flasks in our kitchen labo.
I love In-vitro propagation.
But also, I think that the propagation by the seed is important, too.
Then, I want huge numbers of clones(genetic variability) to continue to
survive in thier native habitats.
Kindest regards
Isao
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