why TC? faster propagation (perhaps 10x) no worry about fungus, pests, etc. long term maintainance of plants (stick 'em in the fridge!) propagating IDENTICAL clones of horticultural varieties Totipotency certain cells have the capacity, when isolated and properly grown, to regernerate a whole plant. Meristem The region in a growing plant where the cells are rapidly dividing Auxin hormone that primarily controls cell elongation inhibits side shoots, produced at apical meristem Cytokinin hormone that primarily stimulates cell division Components of TC growth medium Organic C, H, O AGAR neutral substrate sucrose (C12-H22-O11) provides energy source for cells inositol (C6-H12-O6) simple sugar hormones control growth fungicides control contaminants antibiotics control contaminants antioxidants Inorganic Macro nutrients N leaf growth, chlorophyll, amino acids, proteins, cell membranes P meristem growth, fatty cell membranes, DNA production K cell division, root formation S root development Ca pectin (cell wall glue) vital to meristem growth Mg integral to chlorophyll molecules Fe vital to pigment and chlorophyll formation Inorganic Micro nutrients primary: B Sugar movement within plant Mo Nitrogen Fixation Mn subtle (controls vital enzyme pathways) Cu subtle (controls vital enzyme pathways) Zn subtle (controls vital enzyme pathways) secondary: (the need for these varies by species) Cl Al Na Si Co Simple recipe: 1/8 cup table sugar 1 cup water 1/2 cup stock solution (Miracid diluted 1/4 tsp in 1 Gallon water) 3 drops Miracid stock solution (50/50 by volume) 1/2 inositol tablet (125mg) 1/4 vitamin tablet with thiamin 2 Tablespoons agar flakes For variation, try substituting coconut milk for water. Note: most CP require more dilute concentrations than normal, try reducing proportions of stock solution and sugar until best results are obtained.