Date: Thu, 03 Feb 2000 18:09:34 +0100 From: Fabien ZUNINO <zunino@campus.univ-poitiers.fr> To: cp@opus.hpl.hp.com Message-Id: <aabcdefg345$foo@default> Subject: Triphyophyllum peltatum - Phytochemistry
Hi!
New article about Triphyophyllum peltatum in Phytochemistry:
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Droserone from cell cultures of Triphyophyllum peltatum
(Dioncophyllaceae) and its biosynthetic origin
Gerhard Bringmann, Heiko Rischer, Michael Wohlfarth, Jan Schlauer, Laurent
Ake Assi
Phytochemistry 53 (2000) 339-343
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Abstract
The growth and droserone content of callus cultures of Triphyophyllum
peltatum grown in liquid 1/5 Linsmaier and Skoog
medium was studied. During a lag phase in growth, droserone concentrations
in the medium reached a value of 2.1 mg g-1 fr.
wt. After this maximum value the concentration decreased slightly to 1.8 mg
g-1
fr. wt., while the growth of the calli was enhanced (25% increase in fr.
wt. within 7 days). Plumbagin and isoshinanolone were likewise present in
the dium. By feeding 13 C2 -labelled acetate to the cultures the
biosynthesis of droserone was elucidated. The incorporation of whole C2 -units
unambiguously shows its acetogenic origin and its well in the biosynthetic
scheme suggested for the structurally - and
biogenetically - related naphthylisoquinoline alkaloids.
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Best regards,
Fabien ZUNINO
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