Date: Fri, 25 Feb 2000 00:31:30 +0100 From: "Andreas Wistuba" <andreas@wistuba.com> To: cp@opus.hpl.hp.com Message-Id: <aabcdefg530$foo@default> Subject: AW: tc plant question
Dear Phil,
tissue culture usually refers in the relevant literature to almost
everything coming out of a test tube. You also call it micropropagation.
However, usually the plants are also propagated when they are sarted from
seeds. The seed is only the explant in most cases. This means, that often it
is sufficient to have a few seeds germinating in vitro. FRom these seedlings
propagation s then started. The only cases I amaware of that nurseries do
not propagate but just sow the seeds to grow the seedlings under sterile
conditions are orchids. However, this is mainly done because orchids can
hardly be grown from seeds under non-strile conditions. You also mention the
term meristematic clone. In fact, in the true sense this only refers to a
technique which is rarely used, when taking only the _real_ meristeme, only
consisting of few cells. Most people talking of meristems or meristeme
propagation are actually using shoot tips which also contain
non-meristematic cells, but that's another story...
BTW, most Nepenthes plants that are _propagated_ in vitro were actually
_started_ from seeds sown in vitro under sterile conditions.
Bye
Andreas
-----Urspr\374ngliche Nachricht-----
Von: cp@opus.hpl.hp.com [mailto:cp@opus.hpl.hp.com]Im Auftrag von Phil
Faulisi
Gesendet: Montag, 21. Februar 2000 14:43
An: Multiple recipients of list
Betreff: tc plant question
Greetings members. Is there anyone out there who can enlighten me on the
differences between tc grown nepenthes and seed grown plants. I hear so
many different opinions on the subject it's hard to get a straight
answer. As far as I have always known, tc was a process of cloning by
way of culturing the apical meristem hence the term "tissue". I hear
from many sources that nepenthes sold as tissue cultured are actually
seed grown in vitro. This really isn't true tissue culture...is it? And
if it isn't, then why is it referred to in this way. I think it is all
very confusing. Plants grown from seed suspended in an agar base are
really just seed grown in very sterile conditions, aren't they. I have
contacted many nurseries around the USA who specialize in all kinds of
plants, non cp, and they have ALL said that 100% of the plants they sell
as tissue cultured are indeed meristematic clones of the parent plant.
They too grow plants from seed in vitro but they don't label these as tc
grown. They sell them as seed grown. It's too confusing. Can anyone who
really knows what is going on share some information please? Thanks very
much.
Phil
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