Date: Thu, 07 Sep 2000 16:17:45 +1000 From: Miguel de Salas <mm_de@postoffice.utas.edu.au> To: cp@opus.hpl.hp.com Message-Id: <aabcdefg2739$foo@default> Subject: TC - Disinfestation of seeds with NaClO
What disinfestation protocols have people used successfully on CP seeds?
Is it better to leave them on more dilute solution longer or more
concentrated for a shorter time? I imagine concentrated solutions are more
likely to fry the seeds.
I have used 10% Sodium hypochlorite (NaClO?) for 8 minutes on Sarracenia
seed, but it did not germinate after 3 months. However, I got absolutely no
contamination in 20 tubes I inoculated, which leads me to believe two
things: my laminar flow cabinet works and I probably burned the seed. Seed
of the same batch stratified for 6 weeks and sown is providing good
germination.
When we come to Drosera seed, which is much finer, I would like to know
what people have found a good tradeoff between good germination and a
reasonable disinfestation success.
Cheers!
Miguel de Salas
mailto:mm_de@postoffice.utas.edu.au
School of Plant Science,
University of Tasmania,
GPO Box 252-55, Hobart,
Tasmania, Australia, 7001.
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