Re: TC - Disinfestation of seeds with NaClO

From: Phil Wilson (cp@pwilson.demon.co.uk)
Date: Thu Sep 07 2000 - 05:08:19 PDT


Date: Thu, 7 Sep 2000 13:08:19 +0100
From: Phil Wilson <cp@pwilson.demon.co.uk>
To: cp@opus.hpl.hp.com
Message-Id: <aabcdefg2743$foo@default>
Subject: Re: TC - Disinfestation of seeds with NaClO

Miguel,

>
>When we come to Drosera seed, which is much finer, I would like to know
>what people have found a good tradeoff between good germination and a
>reasonable disinfestation success.
>
I think the method used by the micropropagation unit at Kew Gardens for
fine seed was to make a small envelope of filter paper. Put the fine
seed into the envelope and seal it with a metal staple. The package was
put into a flask containing the solution and put onto a magnetic
stirrer. I think one of the advantages with this method is that not only
is it easier to remove the seed from the solution but several batches of
seed can be sterilised at once.

Regards,
Phil Wilson
Email: cp@pwilson.demon.co.uk
Web Site: www.pwilson.demon.co.uk



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