Nitrogen uptake

From: Andy Falshaw (A.Falshaw@irl.cri.nz)
Date: Tue Jan 02 2001 - 12:34:56 PST


Date: Wed, 8 Jan 1997 13:28:48 GMT+1200
From: A.Falshaw@irl.cri.nz (Andy Falshaw)
To: cp@opus.hpl.hp.com
Message-Id: <aabcdefg103$foo@default>
Subject: Nitrogen uptake

The usual way would be to use radiolabelled stuff, or a stable
isotope and gcms. i don't think you can get radio nitrogen
How about feeding the plant 14C labelled amino acids? And assuming
that where the 14c goes is where the N goes?
Andy
> *SNIP! I was asked by an ecologist friend how she might measure
> the nitrogen budget of a carnivorous pitcher plant. She wants to
> know how efficiently nitrogen-containing compounds from the
> decaying/digesting prey in the pitcher are absorbed and utilized
> by the plant. *SNIP!*
> > This was snipped from a missive from a prof at my alma mater; he
> suggests that labeled nitrogen is not viable (their NMR is only 80 Mhz),
> and that GC-MS would be a bear due to sample workup.
> Anybody have any suggested techniques for determining nitrogen
> uptake in Sarracenias?
>
> -AJHicks
>Wonk
> Socorro, NM



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