Re: Nitrogen uptake

From: Greg Long (GLong@olivet.edu)
Date: Wed Jan 08 1997 - 10:16:20 PST


Date: Wed, 8 Jan 1997 12:16:20 -0600
From: "Greg Long" <GLong@olivet.edu>
To: cp@opus.hpl.hp.com
Message-Id: <aabcdefg117$foo@default>
Subject: Re: Nitrogen uptake

How about a probe that bind to nitrogen and IS measurable by a
feasible method? I measure intracellular calcium in bone cells by
looking at a flourinated Ca "probe" using NMR. I have never used a nitrogen
probe but something like that "might" work.

> *SNIP!
> I was asked by an ecologist friend how she might measure the nitrogen budget
> of a carnivorous pitcher plant. She wants to know how efficiently
> nitrogen-containing compounds from the decaying/digesting prey in the pitcher
> are absorbed and utilized by the plant.
> *SNIP!*
>
> This was snipped from a missive from a prof at my alma mater; he
> suggests that labeled nitrogen is not viable (their NMR is only 80 Mhz),
> and that GC-MS would be a bear due to sample workup.
> Anybody have any suggested techniques for determining nitrogen
> uptake in Sarracenias?
>
> -AJHicks
> Wonk
> Socorro, NM
Gregory J. Long, Ph.D.
Department of Biology
Olivet Nazarene University
Kankakee, IL 60901

phone: (815) 939-5065
fax: (815) 939-5071
email: glong@olivet.edu



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