Date: Fri, 14 Feb 1997 00:54:40 GMT From: Peter Cole <carnivor@flytrap.demon.co.uk> To: cp@opus.hpl.hp.com Message-Id: <aabcdefg602$foo@default> Subject: Re: Tissue Culture help needed
"Carl Mazur" <cherryhillcp@freenet.hamilton.on.ca> writes:
...
> Carl Mazur here, so it looks like you are into TC. Neat. The head of
> Science department where I teach and myself are trying our hands at doing
> TC work with VFTs. We are just waiting to get our fans installed on our
> Laminar flow hood (home made none the less). We have all our chemicals,
> and flasks, our media and tools, we're ready to go. The only question I
> have left unanswered is "what do I use a transplant medium"? You see, we
> plant to start the seeds in vitro in 1/4 strength MS media. Then when
> ready, I plan to clone by division when I transplant the seedlings. What
> sort of chemicals do I add into the transplant media to promote root
> production? Or can I use the same media formula as I used to germinate the
> seedlings.
With Drosera, and Pinguicula certainly - I culture mine on either
1/5 strength MS or 2/3 strength Knudson's 'C', both with 20 g/L
sucrose and MS vitamins, and they root well for the most part.
Tuberous spp. can be a bit slow to root, but a pinch of rooting
powder in the medium does the trick (there's a nice scientific
approach :)
Dionaea will work in plain MS, but is faster and roots better with
the media supplements described on Rick Walker's TC slideshow.
If you get leafy growth and no roots IV, try reculturing with
1/5 (or 1/4) strength MS with 1mg/L kinetin and only 5-10 g/L
sucrose (certainly works for Cephalotus anyway.) But you should
find it roots well enough without this if you use Casein, 2iP, etc.
as described in the TC slideshow.
Don't do this until you have a nice little plantlet though as the
leafy growth can go a bit stringy with the high cytokinin level.
If you haven't got kinetin I'd expect BAP or similar to do the trick.
Hope this helps,
Peter
snail:Peter Cole,17 Wimmerfield Cr. :: mailto:carnivor@flytrap.demon.co.uk
Killay, SWANSEA SA27BU,WALES,UK :: http://www.flytrap.demon.co.uk/
vox:+44 1792 205214 :: Carnivorous plants & seeds for sale
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