tc of VFT

From: kamikaze (011114@hillstrath.on.ca)
Date: Wed Nov 19 1997 - 10:53:48 PST

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Date:          Wed, 19 Nov 1997 14:53:48 -0400
From: "kamikaze" <011114@hillstrath.on.ca>
To: cp@opus.hpl.hp.com
Message-Id: <aabcdefg4425$foo@default>
Subject:       tc of VFT

Dear list,

I recently tissue cultured some VFT petiole cuttings. I immersed the
cuttings in isopropyl alcohol for 3 minutes and then left them in
hydrogen peroxide for another 3 minutes. Today, after two days, I
checked up on the plants and found that they were turning an
unhealthy brown hue. I suspect that I might have 'over sterilized'
the plants. Does anyone know which substances are most effective in
sterilization?

thanks,

adwait kulkarni

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