Re: tc of VFT

From: Jessee G. Soler (jgsoler@earthlink.net)
Date: Wed Nov 19 1997 - 12:50:17 PST

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Date: Wed, 19 Nov 1997 12:50:17 -0800
From: "Jessee G. Soler" <jgsoler@earthlink.net>
To: cp@opus.hpl.hp.com
Message-Id: <aabcdefg4426$foo@default>
Subject: Re: tc of VFT

At 12:11 PM 11/19/97 -0800, you wrote:
>Dear list,
>
>I recently tissue cultured some VFT petiole cuttings. I immersed the
>cuttings in isopropyl alcohol for 3 minutes and then left them in
>hydrogen peroxide for another 3 minutes. Today, after two days, I
>checked up on the plants and found that they were turning an
>unhealthy brown hue. I suspect that I might have 'over sterilized'
>the plants. Does anyone know which substances are most effective in
>sterilization?
>
>thanks,
>
>adwait kulkarni
>

A 10:1 water/bleach ..
More later..
J

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