Date: Fri, 20 Feb 1998 21:19:28 -0800 From: Craig S Gardner <cgardner@jps.net> To: cp@opus.hpl.hp.com Message-Id: <aabcdefg689$foo@default> Subject: Re: tissue culture
I would agree with Peter that bleach is better than alcohol for
sterilizing work surfaces and chamber. I had a lot of hit and miss when
I used alcohol but now that I use bleach (10 percent) I have very little
contamination you might also try PPM links to info and ordering info are
at Carol Stiff's Home page at
http://www.home.turbonet.com/sati/tcinfo.htm. I have just started to
experiment with PPM but it looks very promising so far.
>Better to sterilise the aquarium immediately prior to
>culturing. I prefer to use diluted bleach (same as for plant
>tissue or stronger,) for sterilising - mist or swab all interior
>surfaces and leave for 20 mins. Then just blot lightly to absorb
>excess (it will do no harm to leave the surfaces damp with bleach,
>but you don't want it dripping into the flasks!)
-- Happy GrowingCraig S. Gardner 173 Perry St. Ukiah, Ca. 95482 USA (707)462-5331 (707)468-1175
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