Late Reply D. regia TC

From: Kevin Snively (ksnive@premier1.net)
Date: Wed Feb 25 1998 - 17:04:45 PST


Date: Wed, 25 Feb 1998 17:04:45 -0800 (PST)
From: Kevin Snively <ksnive@premier1.net>
To: cp@opus.hpl.hp.com
Message-Id: <aabcdefg755$foo@default>
Subject: Late Reply D. regia TC


>Date: Mon, 16 Feb 1998 01:14:49 -0800
>From: "Jens Rotthauwe" <uzsb09@uni-bonn.de>
>Subject: Tissue Culture

>Hello TC - experts !
>I have managed to establish some cp (D.regia, D.petiolaris,
>D.falconeri, Da.californica ...) from seeds in vitro.
>They are doing fine but so far I have failed to multiply them.
>I have tried cuttings but they always died after a while.
>If you have any advice or know some tricks please let me know.

Dear Jens
        I'm no TC - expert but I have sucessfully done cuttings
of D. regia once and will share that experence with you hoping
it will help. 3 summers ago I had a mature plant taking up
bench space in my greenhouse. Late in the fall I decided to
transplant it into a hanging basket so it would be closer to its
mate for pollination purposes the following spring and to reclaim
the bench space. During repoting several large roots broke off
and not wanting to waste the material I potted it up and left
it on one of the lower shelves for the winter. High temps were in
the low 50 Deg. F range lows 34 Deg. F avg about 40. Winter is
longish and dark here at 48 deg. North. Late in the spring (about
6 months later I had about 1/2 Doz. small plants from the largest
of the roots. I had tried to make cuttings before this with
no sucess under warmer conditions. I've not tried to make any
since. The parents are doing fine.

Kevin Snively
Everett Washington
U.S.A.



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