Date: Wed, 10 Mar 1999 19:16:08 -0500 From: Joe Cumbee <acumbee@surfsouth.com> To: cp@opus.hpl.hp.com Message-Id: <aabcdefg744$foo@default> Subject: Re: Sterilization of explants. Help
I use a 35ml/ per liter PPM solution to soak seeds and explants in with good
results. visit http://www.home.turbonet.com/kitchenculture/
to find out about PPM.
Eric Cumbee
http://www.mktechnology.com/ericnews.htm
Marin Martiskovich wrote:
> Hello all. I am having problems with sterilization of explants from
> Venus Fly Traps, that I am trying to tissue culture. From what I have read
> on the subject most people seem to recommend first alcohol dipping for a
> minute or two and then a 10% bleach solution for ten minutes. When I attempt
> to do this I notice a purplish pigment leeching out of the explant much like
> someone else experienced with seeds, except that I do not have any luck. The
> explants look slightly limp, like steamed greens, but lighter in colour and
> then they turn black on the medium within a day or so. Should I attempt to
> reduce time in bleach solution or is 10 minutes at 10% basically essential
> to sterilize plant material. Also has anyone attempted to use anything else
> to sterilize explants with success? Possibly Dettol, triclosan, or any other
> antibacterial compound.
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